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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1713

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

N-Phenyl-2-naphthylamine

0.5

0.32

0.25

Saline/EtHO 1:1

Human

Full Thickness Skin

Abdomen, thigh, upper arm

0.64

Static

0.9 NaCl

Skin

GCMS

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

NOTES

Highest concn tested. CAUTION-undetectable probablly because of low solubility in receptor fluid.

REFERENCE

Percutaneous absorption of aromatic amines – A contribution for human health risk assessment (2008) Food Chem.Toxicol., 46 :1960-1968