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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1714

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Nickel

0.027

0.0082

synthetic sweat pH 6.5

Human

Full Thickness Skin

abdomen

3.29

Static

phosphate buffered 0.9% NaCl in high purity water

Receptor Fluid

GFAAS

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.0165	0.0001	0.00061	5E-05	14.56

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0165

0.0001

0.00061

5E-05

14.56

NOTES

SD reported. SEMs have been calculated. Application was 50 mg/ml of powder (APS 2.2-3.0 um), concentration and loading are for ions in filtered donor phase

REFERENCE

In vitro percutaneous absorption of metal compounds (2007) Toxicol. Lett., 170 :49-56