IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1715
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Chromium
0
1
0
synthetic sweat pH 6.5
Human
Full Thickness Skin
abdomen
3.29
Static
phosphate buffered 0.9% NaCl in high purity water
32
Receptor Fluid
24
24
2
GFAAS
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0
0
0
NOTES
Application was 50 mg/ml chromium powder (ASP<10 um). No ion content detected in filtered donor phase.
REFERENCE
In vitro percutaneous absorption of metal compounds (2007) Toxicol. Lett., 170 :49-56
