IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1718
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
MDA
5
0.02
0.313
50% ethanol:water
Human
Full Thickness Skin
Abdomen
0.32
Flow-through
0.9%NaCl
32
Skin
24
24
1
LSC
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
16.2
3
11.35
1.36
0.00227
0.00027
4.15
NOTES
% absorbed values are for 8 hrs.
REFERENCE
Percutaneous penetration and genotoxicity of 4,4′-methylenedianiline through rat and human skin in vitro (2004) Toxicology, 196 :65-75
