EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 172

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

2-Phenoxyethanol

35.372

0.01

0.553

Methanol

Human

Dermatomed to 0.33mm

Breast/Abdomen/Leg

0.64

Flow-through

MEM & penicilllin/streptomycin + CO2

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
						67.8		59.3	12.1			0.001337	3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

67.8

59.3

12.1

0.001337

NOTES

Volume added was given as 2560 nmol or 1.32mCi. Sp Activity was 3.74mCi/mg. The rats were Male Wistar Rats and hair was removed from the dorsal skin with clippers. The results were given as +/- SEM. I have converted this to SD for this database.

REFERENCE

Percutaneous Penetration Of 2-Phenoxyethanol Through Rat And Human Skin (1997) Food Chem.Toxicol., 35 :1009-1016