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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1720

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
MDA50.020.31350% ethanol:waterHumanFull Thickness Skinabdomen0.32Flow-through0.9% NaCl32Skin481LSC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        18.65.8    3.1

NOTES

% absorbed values are for 8 hr. Flux and kp not sig different from 24 hr exposure.

REFERENCE

Percutaneous penetration and genotoxicity of 4,4′-methylenedianiline through rat and human skin in vitro (2004) Toxicology, 196 :65-75