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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 176

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Testosterone

0.00289

2.5

Water

Human

Isolated Epidermis

Not Given

Static

Aq. Penicillin/streptomycin

Not Given

Radiolabelled

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.00115		0.0004		2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00115

0.0004

NOTES

100 micro curies of the steroid was used. Further experimental method can be derived from other papers. J. Invest. Derm. 45:334 (1966), J. Invest. Derm 48:79 (1967) J. Invest Derm. 49:5S2 (1967)

REFERENCE

Percutaneous Absorption Of Steroids (1969) J. Investig. Dermatol., 52 :63-70