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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 185

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

17a-Hydroxyprogesterone

0.0033

2.5

Water

Human

Isolated Epidermis

Not Given

Static

Aq. Penicillin/streptomycin

Not Given

Radiolabelled

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.00198		0.0006

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00198

0.0006

NOTES

100 micro curies of the steroid was used. Further experimental method can be derived from other papers. J. Invest. Derm. 45:334 (1966), J. Invest. Derm 48:79 (1967) J. Invest Derm. 49:5S2 (1967)

REFERENCE

Percutaneous Absorption Of Steroids (1969) J. Investig. Dermatol., 52 :63-70