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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 19

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

0.05

0.24

Acetone

Human

Full Thickness Skin

Abdomen

3.14

Static

20% aq. Human albumin

Receptor Fluid

Reference (will check)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								3.9	2.5				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

3.9

2.5

NOTES

Female skin was used. No steady state flux was achieved. Results were given as 942 +/- 2159 ng/cm2 (SE) I have converted these to percentages and from SE to SD. The researchers also performed the experiment under increased atmospheric pressure absorption is increased 1.8x. An integrity test was performed with methylene blue.

REFERENCE

Effect Of Pressure On In Vitro Percutaneous Absorption Of Caffeine (1993) Acta Derm. Venereol., 73 :200-202