EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 194

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Testosterone0.04960.050.00142BenzeneHumanFull Thickness SkinAbdomen1.744StaticSaline Solution37 14.414.41Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        0.760.16   3 

NOTES

The cell had a ground glass lid that was refitted after the compound had been added. Cadaver skin was used. 0.5 microcuries of the radiolabelled compound was added. They also measured the distribution between the epidermis and dermis. Though they included the amount left on the surface in the epidermis column. Testosterone is highly lipophillic and they used an aqueous receptor fluid with full-thickness skin which may have affected the results. I calculated the mean and SD from the raw penetration data

REFERENCE

In Vitro Human Percutaneous Penetration Of Benzyl Alcohol And Testosterone: Epidermal-Dermal Retention (1970) J. Investig. Dermatol., 54 :386-394