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In Vitro Study No: 198

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Beta-Estradiol6.1Infinite Dose 5% Oleic Acid in 75% EthanolHumanDermatomed to 0.5mmAbdomen Static5% Oleic Acid in 75% Ethanol32Water Bath60601Radiolabelled (3H)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          40.736.40.015 

NOTES

As an infinite dose was added it is as though the cells were occluded. Beta-Estradiol Solutions were prepared by adding an excess of the drug to the vehicle. These solutions were allowed to equilibriate over 3 days and then spiked with radiolabelled drug at 0.1microCi/mg. The specific radioactivity of the drug was 60Ci/mmol. The results were given as +/- SE. I have changed this to SD for the database

REFERENCE

Disparity Of In Vitro And In Vivo Oleic Acid-Enhanced Beta-Estradiol Percutaneous Absorption Across Human Skin (1993) Pharmaceut. Res., 10 :1745-1750