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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 199

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Beta-Estradiol

6.1

Infinite Dose

5% Oleic Acid in 75% Ethanol

Human

Dermatomed in 0.5mm

Abdomen

Static

75% Ethanol

Water Bath

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.0033	5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0033

NOTES

As an infinite dose was added it is as though the cells were occluded. Beta-Estradiol Solutions were prepared by adding an excess of the drug to the vehicle. These solutions were allowed to equilibriate over 3 days and then spiked with radiolabelled drug at 0.1microCi/mg. The specific radioactivity of the drug was 60Ci/mmol

REFERENCE

Disparity Of In Vitro And In Vivo Oleic Acid-Enhanced Beta-Estradiol Percutaneous Absorption Across Human Skin (1993) Pharmaceut. Res., 10 :1745-1750