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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 2

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Paclobutrazol0.05880.60.017350% aq. EthanolHumanFull Thickness SkinAbdomen2.04Static50% aq. Ethanol32Water Bath72721HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
             918

NOTES

Prior to the experiment the skin was exposed to the receptor fluid for 18h to ensure that it was fully hydrated. Skin was occluded with parafilm during the experiment to prevent evaporation. Human Cadaver Skin was used from white women.

REFERENCE

Dermal In Vitro Penetration Of Methiocarb, Paclobutrazol and Pirimicarb (2000) Occup. Environ. Med., 57 :734-737