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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 200

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Phenol

Not Given

Isotonic Phosphate Buffer

Snake

Full Thickness Skin

Dorsal

1.8

Static

Isotonic Phosphate Buffer

Not Given

UV Absorbance

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00523	4	0

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00523

NOTES

Shed snake skins of the black rat snake were used. The donor concentration was 1-3 mg/ml. Penetration was very rapid and there was almost no lag time.

REFERENCE

Use Of Shed Snake Skin As A Model Membrane For In Vitro Percutaneous Penetration Studies: Comparison With Human Skin (1990) Pharmaceut. Res., 7 :1042-1047