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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 201

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

m-Cresol

Not Given

Isotonic Phosphate Buffer

Snake

Full Thickness Skin

Dorsal

1.8

Static

Isotonic Phosphate Buffer

Not Given

UV Absorbance

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00988	4	0

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00988

NOTES

Shed snake skins of the black rat snake were used. The donor concentration was 1-3 mg/ml. Penetration was very rapid and there was almost no lag time.

REFERENCE

Use Of Shed Snake Skin As A Model Membrane For In Vitro Percutaneous Penetration Studies: Comparison With Human Skin (1990) Pharmaceut. Res., 7 :1042-1047