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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 202

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Corticosterone Not Given Isotonic Phosphate BufferSnakeFull Thickness SkinDorsal1.8StaticIsotonic Phosphate Buffer25Not Given36362HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.00010257

NOTES

Shed snake skins of the black rat snake were used. The donor concentration was 1-3 mg/ml. Lag time was between 6 and 8 hours

REFERENCE

Use Of Shed Snake Skin As A Model Membrane For In Vitro Percutaneous Penetration Studies: Comparison With Human Skin (1990) Pharmaceut. Res., 7 :1042-1047