IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 202
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Corticosterone
Not Given
Isotonic Phosphate Buffer
Snake
Full Thickness Skin
Dorsal
1.8
Static
Isotonic Phosphate Buffer
25
Not Given
36
36
2
HPLC
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.000102
5
7
NOTES
Shed snake skins of the black rat snake were used. The donor concentration was 1-3 mg/ml. Lag time was between 6 and 8 hours
REFERENCE
Use Of Shed Snake Skin As A Model Membrane For In Vitro Percutaneous Penetration Studies: Comparison With Human Skin (1990) Pharmaceut. Res., 7 :1042-1047