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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 203

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Mannitol10.5080.2Distilled waterRatFull Thickness SkinDorsal2.54Static0.9% Physiological Saline30Water Bath96961Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.323 0.000323178 

NOTES

Alderley Park Rats, strain Alpk:APfSD were used. The skin was occluded from days 2-4. Steady-state flux was not given in the paper. I have calculated it from the Kp and the chemical concentration.

REFERENCE

The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887