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In Vitro Study No: 204

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Mannitol10.5080.2Distilled waterRatFull Thickness SkinDorsal2.54Static0.9% Physiological Saline30Water Bath96961Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.289 0.000289150 

NOTES

Sprague-Dawley Rats (strain Crl:CD(SD)BR) were used. The skin was occluded from days 2-4. Steady-state flux was not given in the paper. I have calculated it from the Kp and the chemical concentration.

REFERENCE

The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887