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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 205

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Mannitol

0.508

0.2

Distilled water

Rat

Isolated Epidermis

Dorsal

2.54

Static

0.9% Physiological Saline

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.23		0.00023	30

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.23

0.00023

NOTES

Alderley Park Rats, strain Alpk:APfSD were used. The skin was occluded from days 2-4. Steady-state flux was not given in the paper. I have calculated it from the Kp and the chemical concentration.

REFERENCE

The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887