IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 206
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Mannitol
1
0.508
0.2
Distilled water
Rat
Isolated Epidermis
Dorsal
2.54
Static
0.9% Physiological Saline
30
Water Bath
96
96
1
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.089
8.9E-05
22
NOTES
Sprague-Dawley Rats (strain Crl:CD(SD)BR) were used. The skin was occluded from days 2-4. Steady-state flux was not given in the paper. I have calculated it from the Kp and the chemical concentration.
REFERENCE
The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887