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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 207

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Water

1.016

0.9% Physiological Saline

Rat

Full Thickness Skin

Dorsal

2.54

Static

0.9% Physiological Saline

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.00143	178

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.00143

178

NOTES

Sp Act. Of the tritiated water was 270mCi/mmol. This was diluted to give a final concentration of 2.5microCi/ml. Alderley Park Rats, strain Alpk:APfSD were used. The skin was occluded from days 2-4.

REFERENCE

The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887