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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 209

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Water 1.016 0.9% Physiological SalineRatIsolated EpidermisDorsal2.54Static0.9% Physiological Saline30Water Bath96961Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.0011630 

NOTES

Sp Act. Of the tritiated water was 270mCi/mmol. This was diluted to give a final concentration of 2.5microCi/ml. Alderley Park Rats, strain Alpk:APfSD were used. The skin was occluded from days 2-4.

REFERENCE

The Influence Of Different Strains And Age On In Vitro Rat Skin Permeability To Water And Mannitol (1992) Pharmaceut. Res., 9 :884-887