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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 218

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

Not Given

0.004

Not Given

Pig

Dermatomed Skin

Dorsal Thorax

0.8

Flow-through

Aerated Tissue Culture Medium

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								18	3				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

NOTES

The cell had a vapor trap above the dosing area. They did not mention how they achieved the concentrations used. No vehicle was mentioned. The radioactive dose applied was 0.05microCiThey stated that the proceedures were carried out in accordance to published proceedures: hawkins GS, et al. Fundam. Appl. Toxicol. (1984). 4 S133-S144. WILL CHECK

REFERENCE

Influence Of Skin Source, Penetration Cell Fluid, And Partition Coefficient On In Vitro Skin Penetration (1986) J. Pharm. Sci., 75 :378-381