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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 223

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Methyl nicotinate

100

17.48

Water

Human

Isolated Epidermis

Abdomen/Breast

5.72

Static

isotonic PBS and NaCl

Not Given

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										309		0.00309	5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

309

0.00309

NOTES

The top of the chamber was sealed to prevent evaporation. The Kp was given as log Kp, cm/h.10 to the power of 3 which was 0.49 I have converted it to Kp for the purposes of this database.

REFERENCE

Percutaneous Absorption Of Nicotininc Acid Derivatives In Vitro (1991) J. Pharm. Sci., 80 :54-56