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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 226

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzyl nicotinate

0.86

0.15

Water

Human

Isolated Epidermis

Abdomen/Breast

5.72

Static

isotonic PBS and NaCl

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										13.9		0.016	5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

13.9

0.016

NOTES

The top of the chamber was sealed to prevent evaporation. The Kp was given as log Kp, cm/h.10 to the power of 3 which was1.21. I have converted it to Kp for the purposes of this database.

REFERENCE

Percutaneous Absorption Of Nicotininc Acid Derivatives In Vitro (1991) J. Pharm. Sci., 80 :54-56