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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 23

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Terbinafine

0.01

0.3

0.0012

Propylene glycol

Human

Dermatomed to 0.6mm

Abdomen

2.54

Static

PBS/Ethanol 3:1

Water Bath

reversed-phase HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.01		0.001	3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.01

0.001

NOTES

The flux was actually recorded as <0.01. No integrity test was mentioned. They also measured the concentration of chemical in the skin after the stratum cormeum had been stripped off.. They found this skin concentration to be 175+/-36 micrograms/gram. The Kp was not mentioned in the paper. I calculated it by dividing the steady state flux by the concentration

REFERENCE

Comparison Of Human Skin Or Epidermis Models With Human And Animal Skin In In Vitro Percutaneous Absorption (2001) Int. J. Pharm., 215 :51-56