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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 232

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Butyl nicotinate

1000

0.2

34.96

None

Human

Isolated Epidermis

Abdomen/Breast

5.72

Static

isotonic PBS and NaCl

Not Given

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										79		7.9E-05	5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

7.9E-05

NOTES

The top of the chamber was sealed to prevent evaporation. The compound was applied as a pure liquid. A concentration of 1000mg/ml was assumed, disregarding small errors due to different densities.The Kp was given as log Kp, cm/h.10 to the power of 3 which was-1.1. I have converted it to Kp for the purposes of this database.

REFERENCE

Percutaneous Absorption Of Nicotininc Acid Derivatives In Vitro (1991) J. Pharm. Sci., 80 :54-56