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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 238

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Nicardipine

Water

Human

Dermatomed to 0.3mm

Not Given

Static

0.01M Citric Acid & 0.02M Na2PO4 (pH 5.0)

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										3.76	0.21		3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

3.76

0.21

NOTES

All experiments were conducted with skin samples from the same donor to minimise variation. The concentration was not given but the intrinsic solubility was given as 1.93microg/ml at 25 degrees celsius.

REFERENCE

Effect Of Receiver Fluid pH On In Vitro Skin Flux Of Weakly Ionisable Drugs (1993) Pharmaceut. Res., 10 :986-990