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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 241

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Nicardipine 2 WaterHumanDermatomed to 0.3mmNot Given2Static0.01M Citric Acid & 0.02M Na2PO4 (pH 6.6)32Incubator36362HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.740.17 3 

NOTES

All experiments were conducted with skin samples from the same donor to minimise variation. The concentration was not given but the intrinsic solubility was given as 1.93microg/ml at 25 degrees celsius.

REFERENCE

Effect Of Receiver Fluid pH On In Vitro Skin Flux Of Weakly Ionisable Drugs (1993) Pharmaceut. Res., 10 :986-990