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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 247

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Cortisone

Not Given

Acetone

Rat

Dermatomed to 0.35mm

Not Given

0.64

Static

Saline

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								6.3	2.3				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

6.3

2.3

NOTES

Female Osborne-Mendel rats of 3-6 months of age were used. Further experimental detail is available in Bronaugh et al. J. Invest. Derm (1981) 76;94. The results were given as +/- SEM I have changed this to SD for the database.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies IV: The Flow-Through Diffusion Cell (1984) J. Pharm. Sci., 74 :64-67