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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 250

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Cortisone Not Given PetrolatumRatDermatomed to 0.35mmNot Given0.64StaticSaline32Water Bath241202Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        22.86   5 

NOTES

Female Osborne-Mendel rats of 3-6 months of age were used. Skin was washed at 24h. The results were given as +/- SEM I have changed this to SD for the database.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies IV: The Flow-Through Diffusion Cell (1984) J. Pharm. Sci., 74 :64-67