IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 252
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Cortisone
Not Given
Petrolatum
Rat
Dermatomed to 0.35mm
Not Given
0.64
Flow-through
Saline
32
Skin
24
120
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
20.1
2.7
6
NOTES
Female Osborne-Mendel rats of 3-6 months of age were used. Skin was washed at 24h. The results were given as +/- SEM I have changed this to SD for the database.
REFERENCE
Methods For In Vitro Percutaneous Absorption Studies IV: The Flow-Through Diffusion Cell (1984) J. Pharm. Sci., 74 :64-67