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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 253

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Ethanol

789.3

0.005

10.34

None

Pig

Full Thickness Skin

Back

0.385

Flow-through

PBS, 5% NCS & antibiotics

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
2.22		0.08		0.19	0.05	1.24		0.97	0.42				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2.22

0.08

0.19

0.05

1.24

0.97

0.42

NOTES

Cells complete with skin were left to equilibrate with the receptor solution for 18h before the start of the experiment to ensure that the skin was fully hydrated. Only 2.2% of the applied ethanol was recovered impying that most of it had evaporated.

REFERENCE

Fate Of Ethanol Topically Applied To The Skin (2001) Food Chem.Toxicol., 39 :161-174