EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

University of Newcastle homepage

In Vitro Study No: 254

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Ethanol789.30.00510.34NonePigFull Thickness SkinBack0.385Flow-throughPBS, 5% NCS & antibiotics37Receptor Fluid24241Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
40.03 0.17 1.680.0523.02 21.174.31   3 

NOTES

Cells were occluded with a double laayer of parafilm covered with a glass coverslip. Prior to the experiment, cells complete with skin were left to equilibrate with the receptor solution for 18h before the start of the experiment to ensure that the skin was fully hydrated. Only 40.03% of the applied ethanol was recovered implying that some of it had evaporated at some point during the experiment.

REFERENCE

Fate Of Ethanol Topically Applied To The Skin (2001) Food Chem.Toxicol., 39 :161-174