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In Vitro Study No: 255

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Salicylic acid0.090.10.009Not GivenHumanDermatomed to 0.5mmAbdomen1StaticMEM, Ham's F-12, Sodium Bicarbonate, HEPES & gentamicin sulphate37Incubator12122Radoilabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
92.96.7 0.92.2 32.9 30.71.20.120.1 39

NOTES

The 3 repetitions were all taken from the same skin source -a 55yr old White man. The steady state flux and lag time was calculated from an average of studies 128, 255, 256 and 257

REFERENCE

Skin Penetration And Metabolism: Comparative Evaluation Of Skin Equivalent, Cell Culture And Human Skin (1993) J. Toxicol.:Cutaneous Ocul. Toxicol., 12 :129-138