IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 257
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Salicylic acid
0.09
0.1
0.009
Not Given
Human
Dermatomed to 0.5mm
Not Given
1
Static
MEM, Ham's F-12, Sodium Bicarbonate, HEPES & gentamicin sulphate
37
Incubator
12
12
2
Radoilabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
87
2.5
0.6
1.9
5
3.1
1.8
0.12
0.1
3
9
NOTES
The 3 repetitions were all taken from the same skin source - a 29yr old black man (cadaver). The steady state flux and lag time was calculated from an average of studies 128, 255, 256 and 257
REFERENCE
Skin Penetration And Metabolism: Comparative Evaluation Of Skin Equivalent, Cell Culture And Human Skin (1993) J. Toxicol.:Cutaneous Ocul. Toxicol., 12 :129-138
