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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 266

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dinitrochlorobenzene

0.016

0.5

Acetone

Mouse

Full Thickness Skin

Dorsal

0.64

Flow-through

RPMI-1640 & gentamycin

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
89.3		0	12	51.1	0	61.6		10.5	7.2				36

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

89.3

51.1

61.6

10.5

7.2

NOTES

Balb C mouse skin. 6 different animals were used and 6 cells were used for each animal. The % Penetrated was derived from the % found in the receptor fluid + the % found in the solubilized skin. Results were given as +/- SEM. I have converted these to SD for this database. No dichlorobenzene was detecable in the tape strips of the stratum corneum

REFERENCE

Percutaneous Absorption And Metabolism Of Dinitrochlorobenzene In Vitro (2000) Arch. Toxicol., 74 :