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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 269

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Dinitrochlorobenzene10.0160.025AcetoneRatFull Thickness SkinDorsal0.64Flow-throughRPMI-1640 & gentamycin32Not Given24241Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
76.7  9.614.8 58.8 4412.2   6 

NOTES

Skin was Occluded with Parafilm. Neonatal Rats were used. The % Penetrated was derived from the % found in the receptor fluid + the % found in the solubilized skin. Results were given as +/- SEM. I have converted these to SD for this database.

REFERENCE

Percutaneous Absorption And Metabolism Of Dinitrochlorobenzene In Vitro (2000) Arch. Toxicol., 74 :