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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 271

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Dinitrochlorobenzene

0.016

0.025

Acetone

Rat

Dermatomed to 0.35mm

Dorsal

0.64

Flow-through

RPMI-1640 & gentamycin

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
52.9			3.4	7.6		37.2		29.6	6.1				6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

52.9

3.4

7.6

37.2

29.6

6.1

NOTES

26 day-od rats were used. The % Penetrated was derived from the % found in the receptor fluid + the % found in the solubilized skin. Results were given as +/- SEM. I have converted these to SD for this database.

REFERENCE

Percutaneous Absorption And Metabolism Of Dinitrochlorobenzene In Vitro (2000) Arch. Toxicol., 74 :