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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 288

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
p-Cresol4Infinite Dose Distilled WaterHumanIsolated EpidermisAbdominal2.5StaticDistilled Water25Not Given881Spectrophotometry

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
            0.0175220.266

NOTES

Human Cadaver skin was used. The donor compartment was could hold 30ml but volumes were anything from 9ml upwards. As there is an infinite dose, evaporation does not play a part, it is therefore as though the cell were occluded. Each membrane may have been used for several experiments. Kp was given as 2.92E-4 cm/min

REFERENCE

Permeability Of Human Epidermis To Phenolic Compounds (1977) J. Pharm. Pharmacol., 29 :677-683