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In Vitro Study No: 309

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid0.5080.010.004EthanolPigDermatomed to 1.9mmAnterior right side of back1.27StaticTyrodes Solution37Water Bath50502Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
87  951 71 2013   32

NOTES

Weanlling Yorkshire Pig skin was used.The % Penetrated is derived from adding the amount found in the receptor fluid to the amount found in the skin

REFERENCE

Development Of An In Vitro Model For Determining The Fate Of Chemicals Applied To The Skin (1984) Fundam. Appl. Toxicol., 4 :S133-S144