IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 310
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Malathion
0.508
0.01
0.004
Ethanol
Pig
Dermatomed to 1.9mm
Anterior right side of back
1.27
Static
Tyrodes Solution
37
Water Bath
50
50
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
89
1
42
61
19
14
3
NOTES
Weanlling Yorkshire Pig skin was used.The % Penetrated is derived from adding the amount found in the receptor fluid to the amount found in the skin
REFERENCE
Development Of An In Vitro Model For Determining The Fate Of Chemicals Applied To The Skin (1984) Fundam. Appl. Toxicol., 4 :S133-S144