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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 318

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Diethyl Malonate

0.508

0.01

0.004

Ethanol

Pig

Dermatomed to 1.9mm

Anterior right side of back

1.27

Static

Tyrodes Solution

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
70			10	30		40		10	3				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

NOTES

Weanlling Yorkshire Pig skin was used.The % Penetrated is derived from adding the amount found in the receptor fluid to the amount found in the skin. Total recovery was very low even though an evaporation trap ws used, this is thought to be due to evaporative loss during the application process.

REFERENCE

Development Of An In Vitro Model For Determining The Fate Of Chemicals Applied To The Skin (1984) Fundam. Appl. Toxicol., 4 :S133-S144