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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 32

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Salicylic acid0.010.30.001290% aq. Propylene glycolHairless RatFull ThicknessUnknown2.54StaticPBS/Ethanol 3:132Water Bath48482reversed phase HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          24.292.423 

NOTES

No integrity test was mentioned. They also measured the concentration of chemical remaining in the skin after the stratum cormeum had been removed by stripping with adhesive tape. They found this skin concentration to be 1183+/-78 micrograms/gram. The Kp was not mentioned in the paper. I calculated it by dividing the steady state flux by the concentration

REFERENCE

Comparison Of Human Skin Or Epidermis Models With Human And Animal Skin In In Vitro Percutaneous Absorption (2001) Int. J. Pharm., 215 :51-56