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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 322

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Atrazine

Not Given

0.015

Water

Human

Dermatomed to 0.5mm

Various

Flow-through

Eagles MEM & FBS & gentamycin

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
83	7.3		4.5	12.8		16.3		3.5	1.1				14

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

7.3

4.5

12.8

16.3

3.5

1.1

NOTES

The skin came from 4 different people. The % Absorption was given as 3.5 +/- 0.3 S.E.M I have converted this to SD. The loading was given as 68nmol/cm2. Elsewhere in the paper the loading was stated as 4.6microg/cm2 but I think that this must have been a miscalculation. As 0.015microg is the equivalent of 1.7microCi if the specific activity is 25microCi/micromol

REFERENCE

In Vitro Percutaneous Absorption And Metabolism In Man Of 2-Chlor-ethylamino-isopropylamin-s-triazine (Atrazine) (1993) Arch. Toxicol., 67 :85-91