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In Vitro Study No: 332

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Ketoprofen See Notes23.610% Menthol & Ethanol & Hydroxypropyl celluloseRatFull Thickness SkinNot Given2.54StaticPhosphate Buffer & PEG 40037Receptor Fluid24241HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        67.56.4714.4267.77 30.73

NOTES

The cells were occluded with parafilm. 2g of a 3% Ketoprofen gel was added. Lag time was calculated by llinear regression of the steady state flux.

REFERENCE

Evaluation Of Percutaneous Absorption And Skin Irritation Of Ketoprofen Through Rat Skin: In Vitro And In Vivo Study (2001) Int. J. Pharm., 222 :225-235