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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 341

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

TDCPP

0.00287

0.015

6.7E-05

Acetone

Mouse

Full Thickness Skin

Dorsal

0.64

Flow-through

HEPES-buffered HBSS & 10% FBS

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
95	13.3		9.4	33.1		78.4		45.8	10.9				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

13.3

9.4

33.1

78.4

45.8

10.9

NOTES

Female Hairless Mice were used. Skin integrity was tested with tritiated water. 100pmols of TDCPP were added in total. % penetrated was calculated by adding together the amount found in the receptor fluid to the amount absorbed in the skin.

REFERENCE

In Vitro Dermal Absorption Of Flame Retardant Chemicals (2001) Food Chem.Toxicol., 39 :1263-1270