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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 342

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

TDCPP

0.00057

0.015

1.3E-05

Acetone

Mouse

Full Thickness Skin

Dorsal

0.64

Flow-through

HEPES-buffered HBSS & 10% FBS

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
96.3	1.6		8.9	27.7		84.9		57.2	7.3				8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

96.3

1.6

8.9

27.7

84.9

57.2

7.3

NOTES

Female Hairless Mice were used. Skin integrity was tested with tritiated water. 20pmols of TDCPP were added in total. % penetrated was calculated by adding together the amount found in the receptor fluid to the amount absorbed in the skin. The amount actually abosrbed is 4E-6 mg.

REFERENCE

In Vitro Dermal Absorption Of Flame Retardant Chemicals (2001) Food Chem.Toxicol., 39 :1263-1270