IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 350
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
4-Chlorophenol
0.005-0.01
0.004
Ethanol
Rat
Dermatomed to 0.35mm
Dorsal
0.64
Static
Eagles MEM. 10% BSA
Not Given
72
72
1
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
100
2.3
10.7
98.1
87.4
2.2
7
NOTES
Cells were covered with parafilm to limit evaporation. 6-8 cells were used. I have entered the median into the database. The % penetrated was calculated by adding the % found in the receptor fluid to the % found in the skin.
REFERENCE
Comparative In Vitro Percutaneous Absorption Of P-Substitued Phenols Through Rat Skin Usimg Static And Flow-through Diffusion Systems (1993) Toxicol. Vitro, 7 :221-227