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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 356

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

4-Cyanophenol

0.005-0.01

0.004

Ethanol

Rat

Dermatomed to 0.35mm

Dorsal

0.32

Flow-through

Eagles MEM. 10% BSA

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
100			4.5	8.1		95.4		87.3	5.4				10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

100

4.5

8.1

95.4

87.3

5.4

NOTES

Cells were covered with parafilm to limit evaporation. 9-12 cells were used. The % penetrated was calculated by adding the % found in the receptor fluid to the % found in the skin.

REFERENCE

Comparative In Vitro Percutaneous Absorption Of P-Substitued Phenols Through Rat Skin Usimg Static And Flow-through Diffusion Systems (1993) Toxicol. Vitro, 7 :221-227